PMN-derived netrin-1 attenuates cardiac ischemia-reperfusion injury via myeloid ADORA2B signaling
Abstract
Previous studies implicated the neuronal guidance molecule netrin-1 in attenuating myocardial ischemia-reperfusion injury. However, the tissue-specific sources and receptor signaling events remain elusive. Neutrophils are among the first cells responding to an ischemic insult and can be associated with tissue injury or rescue. We found netrin-1 levels were elevated in the blood of patients with myocardial infarction, as well as in mice exposed to myocardial ischemia-reperfusion. Selectively increased infarct sizes and troponin levels were found in Ntn1loxP/loxP Lyz2 Cre+ mice, but not in mice with conditional netrin-1 deletion in other tissue compartments. In vivo studies using neutrophil depletion identified neutrophils as the main source for elevated blood netrin-1 during myocardial injury. Finally, pharmacologic studies using treatment with recombinant netrin-1 revealed a functional role for purinergic signaling events through the myeloid adenosine A2b receptor in mediating netrin-1–elicited cardioprotection. These findings suggest an autocrine signaling loop with a functional role for neutrophil-derived netrin-1 in attenuating myocardial ischemia-reperfusion injury through myeloid adenosine A2b signaling.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Apr 23, 2021
- Source ID
- 10.1084/jem.20210008
Entities
People
- Boyun Kim
- Catharina Conrad
- Holger K. Eltzschig
- Jae W. Lee
- Jiwen Li
- Nathaniel Berg
- Tingting Weng
- Wei Ruan
- Xiaoyi Yuan
- Xu Zhang
Organizations
- Central South University
- German Research Foundation
- National Institutes of Health
- United States Department of Defense
- University of Münster
- University of Texas Health Science Center at Houston
- Yale University
- Zhejiang University