Synthesis and bioconjugation of alkanethiol-stabilized gold bipyramid nanoparticles
Abstract
Gold bipyramid (GBP) nanoparticles are promising for a range of biomedical applications, including biosensing and surface-enhanced Raman spectroscopy, due to their favorable optical properties and ease of chemical functionalization. Here we report improved synthesis methods, including preparation of gold seed particles with an increased shelf life of ∼1 month, and preparation of GBPs with significantly shortened synthesis time (< 1 h). We also report methods for the functionalization and bioconjugation of the GBPs, including functionalization with alkanethiol self-assembled monolayers (SAMs) and bioconjugation with proteins via carbodiimide cross-linking. Binding of specific antibodies to the nanoparticle-bound proteins was subsequently observed via localized surface plasmon resonance sensing. Rabbit IgG and goat anti-Rabbit IgG antibodies were used as a model system for antibody-antigen interactions. As-synthesized, SAM-functionalized, and bioconjugated bipyramids were characterized using scanning electron microscopy, UV–vis spectroscopy, zeta potential, and dynamic light scattering.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Mar 09, 2021
- Source ID
- 10.1088/1361-6528/abe823
Entities
People
- Kathryn M Mayer
- Leeana Tovias
- Mohammad M Abdul-moqueet
- Priscilla Lopez
Organizations
- Army Research Office
- National Institute of General Medical Sciences