Biomanufacturing organized collagen-based microfibers as a Tissue ENgineered Device (TEND) for tendon regeneration
Abstract
Approximately 800, 000 surgical repairs are performed annually in the U.S. for debilitating injuries to ligaments and tendons of the foot, ankle, knee, wrist, elbow and shoulder, presenting a significant healthcare burden. To overcome current treatment shortcomings and advance the treatment of tendon and ligament injuries, we have developed a novel electrospun Tissue ENgineered Device (TEND), comprised of type I collagen and poly(D,L-lactide) (PDLLA) solubilized in a benign solvent, dimethyl sulfoxide (DMSO). TEND fiber alignment, diameter and porosity were engineered to enhance cell infiltration leading to promote tissue integration and functional remodeling while providing biomechanical stability. TEND rapidly adsorbs blood and platelet-rich-plasma (PRP), and gradually releases growth factors over two weeks. TEND further supported cellular alignment and upregulation of tenogenic genes from clinically relevant human stem cells within three days of culture. TEND implanted in a rabbit Achilles tendon injury model showed new in situ tissue generation, maturation, and remodeling of dense, regularly oriented connective tissue in vivo. In all, TEND’s organized microfibers, biological fluid and cell compatibility, strength and biocompatiblility make significant progress towards clinically translating electrospun collagen-based medical devices for improving the clinical outcomes of tendon injuries.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Feb 24, 2021
- Source ID
- 10.1088/1748-605x/abb875
Entities
People
- Amrita Dasgupta
- Anna Bulysheva
- Bill Simon Dpm
- Hilary Wriggers
- Kelly Coughenour
- Kevin Bonner
- Michael P Francis
- Nardos Sori
- Nathan Kemper
- Nick Thayer
- Rodriguez Dvm Mario
- Samuel Adams Md
- Seth Polk
- Stella Petrova
- Steven Arnoczky Dvm
- Yas Maghdouri-White
Organizations
- Defense Advanced Research Projects Agency