Plug-and-play adaptive optics for two photon high-speed volumetric imaging
Abstract
To understand brain functions, it is important to study functional connectivity among stereoscopically distributed neurons. Since the brain is composed of 3D neuron networks, volumetric imaging with high spatiotemporal resolution is highly desirable. Two-photon microscopy (2PM) conveniently offers 3D tissue imaging with sub-micrometer resolution based on its intrinsic optical sectioning and deep penetration capabilities. However, the main challenge lies in the volumetric imaging speed and contrast reduction in deep tissue due to aberration. In this study, we integrate a tunable acoustic gradient lens and a plug-and-play adaptive-optics lens into 2PM. The former provides ∼100 kHz axial scan rate, achieving volumetric imaging rate in 1–10 Hz range, while the latter enhances image contrast by nearly two-fold in deep brain regions via correcting both systematic and sample aberrations. The combination offers a practical approach toward high-speed, high-contrast optical volumetric imaging of brain tissues.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Apr 01, 2022
- Source ID
- 10.1088/2515-7647/ac6120
Entities
People
- Chang-ling Chung
- Jye-chang Lee
- Jyun-yi Lin
- Ming-Kai Pan
- Shi-wei Chu
- Stefano Bonora
- Ting-chen Chang
- Tommaso Furieri
- Yi-fan Chen
Organizations
- Air Force Research Laboratory
- National Science and Technology Council
- National Taiwan University
- Office of Naval Research Global