AmpUMI: design and analysis of unique molecular identifiers for deep amplicon sequencing
Abstract
Unique molecular identifiers (UMIs) are added to DNA fragments before PCR amplification to discriminate between alleles arising from the same genomic locus and sequencing reads produced by PCR amplification. While computational methods have been developed to take into account UMI information in genome-wide and single-cell sequencing studies, they are not designed for modern amplicon-based sequencing experiments, especially in cases of high allelic diversity. Importantly, no guidelines are provided for the design of optimal UMI length for amplicon-based sequencing experiments.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jun 27, 2018
- Source ID
- 10.1093/bioinformatics/bty264
Entities
People
- Daniel E. Bauer
- Kendell Clement
- Luca Pinello
- Rick Farouni
Organizations
- Boston Children's Hospital
- Broad Institute
- Burroughs Wellcome Fund
- Defense Advanced Research Projects Agency
- Doris Duke Charitable Foundation
- Harvard Medical School
- Harvard Stem Cell Institute
- Massachusetts General Hospital
- National Heart, Lung, and Blood Institute
- National Human Genome Research Institute
- National Institute of Diabetes and Digestive and Kidney Diseases