SwabExpress: An End-to-End Protocol for Extraction-Free COVID-19 Testing
Abstract
The urgent need for massively scaled clinical testing for SARS-CoV-2, along with global shortages of critical reagents and supplies, has necessitated development of streamlined laboratory testing protocols. Conventional nucleic acid testing for SARS-CoV-2 involves collection of a clinical specimen with a nasopharyngeal swab in transport medium, nucleic acid extraction, and quantitative reverse-transcription PCR (RT–qPCR). As testing has scaled across the world, the global supply chain has buckled, rendering testing reagents and materials scarce. To address shortages, we developed SwabExpress, an end-to-end protocol developed to employ mass produced anterior nares swabs and bypass the requirement for transport media and nucleic acid extraction.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Oct 07, 2021
- Source ID
- 10.1093/clinchem/hvab132
Entities
People
- Ashley E Kim
- Barry R. Lutz
- Beth K Martin
- Brian Pfau
- Caitlin R Wolf
- Christina M. Lockwood
- Cécile Viboud
- Deborah A. Nickerson
- Denise J. McCulloch
- Elisabeth Brandstetter
- Eric Q. Konnick
- Evan Mcdermot
- Helen Y. Chu
- Janet A Englund
- Jase Gehring
- Jason S. Debley
- Jay Shendure
- Jeremy Stone
- Jordan Opsahl
- Kaitlyn A. Barrow
- Katrina Van Raay
- Lea M Starita
- Lucille M. Rich
- Luis Gamboa
- Mark J Rieder
- Matthew P. Thompson
- Melissa Truong
- Michael Boeckh
- Michael Famulare
- Misja Ilcisin
- Nahum Smith
- Peter D Han
- Rachel E Geyer
- Sanjay Srivatsan
- Sarah Heidl
- Sarah L Sohlberg
- Seattle Flu Study Investigators
- Shari Cho
- Sriram Kosuri
- Trevor Bedford
- Wei Chen
- Weizhi Zhong
Organizations
- Fred Hutchinson Cancer Center
- Gates Foundation
- Howard Hughes Medical Institute
- National Human Genome Research Institute
- Sanofi Pasteur
- Seattle Children's
- University of California, Los Angeles
- University of Washington