A Composite Recombinant Salivary Proteins Biomarker for Phlebotomus argentipes Provides a Surveillance Tool Postelimination of Visceral Leishmaniasis in India
Abstract
Incidence of visceral leishmaniasis (VL) in the Indian subcontinent (ISC) has declined by more than 95% since initiation of the elimination program in 2005. As the ISC transitions to the postelimination surveillance phase, an accurate measurement of human-vector contact is needed to assure long-term success. To develop this tool, we identified PagSP02 and PagSP06 from saliva of Phlebotomus argentipes, the vector of Leishmania donovani in the ISC, as immunodominant proteins in humans. We also established the absence of cross-reactivity with Phlebotomus papatasi saliva, the only other human-biting sand fly in the ISC. Importantly, by combining recombinant rPagSP02 and rPagSP06 we achieved greater antibody recognition and specificity than single salivary proteins. The receiver operating characteristics curve for rPagSP02 + rPagSP06 predicts exposure to Ph. argentipes bites with 90% specificity and 87% sensitivity compared to negative control sera (P >.0001). Overall, rPagSP02 + rPagSP06 provides an effective surveillance tool for monitoring vector control efforts after VL elimination.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Sep 02, 2022
- Source ID
- 10.1093/infdis/jiac354
Entities
People
- Alvaro Acosta-serrano
- Caryn Bern
- Clair Rose
- Claudio Menenses
- Eva Iniguez
- Fabiano Oliveira
- Georgios Petrellis
- Jesus G. Valenzuela
- Lee Rafuse Haines
- Naomi E. Aronson
- Samantha Herbert
- Samiran Saha
- Shaden Kamhawi
- Sridhar Srikantiah
- Tiago D Serafim
- Tobin Rowland
- Yvonne Gonzalez-rangel
Organizations
- Armed Forces Health Surveillance Center
- Gates Foundation
- Liverpool School of Tropical Medicine
- National Institute of Allergy and Infectious Diseases
- National Institutes of Health
- Uniformed Services University of the Health Sciences
- United States Department of Defense
- University of Antwerp
- University of California, San Francisco
- Visva-Bharati University
- Walter Reed Army Institute of Research