Anti-OspA DNA-Encoded Monoclonal Antibody Prevents Transmission of Spirochetes in Tick Challenge Providing Sterilizing Immunity in Mice
Abstract
We recently developed anti-OspA human immunoglobulin G1 monoclonal antibodies (HuMAbs) that are effective in preventing Borrelia transmission from ticks in a murine model. Here, we investigated a novel approach of DNA-mediated gene transfer of HuMAbs that provide protection against Lyme disease. Plasmid DNA-encoded anti-OspA HuMAbs inoculated in mice achieved a serum antibody concentration of >6 μg/mL. Among mice injected with DNA-encoded monoclonal antibodies, 75%–77% were protected against an acute challenge by Borrelia-infected ticks. Our results represent the first demonstration of employing DNA transfer as a delivery system for antibodies that block transmission of Borrelia in animal models.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Nov 21, 2018
- Source ID
- 10.1093/infdis/jiy627
Entities
People
- Ami Patel
- Aurélie Kern
- David B. Weiner
- Jacqueline Chu
- Kate E. Broderick
- Katherine Sullivan
- Linden Hu
- Mark S. Klempner
- Megan C. Wise
- Rianne Esquivel
- Sangya Agarwal
- Seleeke Flingai
- Yang Wang
- Zachary A. Schiller
Organizations
- Defense Advanced Research Projects Agency
- Inovio Pharmaceuticals
- National Center for Advancing Translational Sciences
- National Institutes of Health
- Tufts University
- Wistar Institute