A replication-linked mutational gradient drives somatic mutation accumulation and influences germline polymorphisms and genome composition in mitochondrial DNA
Abstract
Mutations in mitochondrial DNA (mtDNA) cause maternally inherited diseases, while somatic mutations are linked to common diseases of aging. Although mtDNA mutations impact health, the processes that give rise to them are under considerable debate. To investigate the mechanism by which de novo mutations arise, we analyzed the distribution of naturally occurring somatic mutations across the mouse and human mtDNA obtained by Duplex Sequencing. We observe distinct mutational gradients in G→A and T→C transitions delimited by the light-strand origin and the mitochondrial Control Region (mCR). The gradient increases unequally across the mtDNA with age and is lost in the absence of DNA polymerase γ proofreading activity. In addition, high-resolution analysis of the mCR shows that important regulatory elements exhibit considerable variability in mutation frequency, consistent with them being mutational ‘hot-spots’ or ‘cold-spots’. Collectively, these patterns support genome replication via a deamination prone asymmetric strand-displacement mechanism as the fundamental driver of mutagenesis in mammalian DNA. Moreover, the distribution of mtDNA single nucleotide polymorphisms in humans and the distribution of bases in the mtDNA across vertebrate species mirror this gradient, indicating that replication-linked mutations are likely the primary source of inherited polymorphisms that, over evolutionary timescales, influences genome composition during speciation.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Oct 06, 2021
- Source ID
- 10.1093/nar/gkab901
Entities
People
- Brendan F Kohrn
- David J. Marcinek
- Elizabeth K. Schmidt
- Jeanne Fredrickson
- Jeremy A Whitson
- Kristine A Tsantilas
- Mariya T. Sweetwyne
- Matthew D Campbell
- Michael J. Hipp
- Monica Sanchez-Contreras
- Peter S. Rabinovitch
- Scott R. Kennedy
Organizations
- Congressionally Directed Medical Research Programs
- National Human Genome Research Institute
- National Institute of Diabetes and Digestive and Kidney Diseases
- National Institute on Aging
- University of Washington