KRCC1, a modulator of the DNA damage response

Abstract

The lysine-rich coiled-coil 1 (KRCC1) protein is overexpressed in multiple malignancies, including ovarian cancer, and overexpression correlates with poor overall survival. Despite a potential role in cancer progression, the biology of KRCC1 remains elusive. Here, we characterize the biology of KRCC1 and define its role in the DNA damage response and in cell cycle progression. We demonstrate that KRCC1 associates with the checkpoint kinase 1 (CHK1) upon DNA damage and regulates the CHK1-mediated checkpoint. KRCC1 facilitates RAD51 recombinase foci formation and augments homologous recombination repair. Furthermore, KRCC1 is required for proper S-phase progression and subsequent mitotic entry. Our findings uncover a novel component of the DNA damage response and a potential link between cell cycle, associated damage response and DNA repair.

Document Details

Document Type
Pub Defense Publication
Publication Date
Oct 16, 2022
Source ID
10.1093/nar/gkac890

Entities

People

  • Anindya Dey
  • Elangovan Thavathiru
  • Fiifi Neizer-ashun
  • Priyabrata Mukherjee
  • Resham Bhattacharya
  • Shailendra Kumar Dhar Dwivedi
  • Susan Patricia Lees-miller
  • William L Berry

Organizations

  • National Cancer Institute
  • United States Department of Defense
  • University of Calgary
  • University of Oklahoma Health Sciences Center

Tags

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics

Technology Areas

  • Biotechnology