Low RNA stability signifies strong expression regulatability of tumor suppressors
Abstract
RNA expression of a gene is determined by not only transcriptional regulation, but also post-transcriptional regulation of RNA decay. The precise regulation of RNA stability in the cell plays an important role in normal development. Dysregulation of RNA stability can lead to diseases such as cancer. Here we found tumor suppressor RNAs tended to decay fast in normal cell types when compared with other RNAs. Consistent with a negative effect of m6A modification on RNA stability, we observed preferential deposition of m6A on tumor suppressor RNAs. Moreover, abundant m6A and fast decay of tumor suppressor RNAs both tended to be further enhanced in prostate cancer cells relative to normal prostate epithelial cells. Further, knockdown of m6A methyltransferase METTL3 and reader YTHDF2 in prostate cancer cells both posed stronger effect on tumor suppressor RNAs than on other RNAs. These results indicated a strong post transcriptional expression regulatability mediated by abundant m6A modification on tumor suppressor RNAs.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Oct 13, 2023
- Source ID
- 10.1093/nar/gkad838
Entities
People
- Ivone Bruno
- Jie Lv
- Kaifu Chen
- Kulandaisamy Arulsamy
- Lili Zhang
- Qi Cao
- Sahana Suresh Babu
- Xinlei Gao
- Yanqiang Li
- Yi Yang
Organizations
- American Cancer Society
- Boston Children's Hospital
- Harvard Medical School
- Harvard University
- Houston Methodist Hospital
- National Institutes of Health
- Northwestern University
- United States Department of Defense