Acetabular Reaming Is a Reliable Model to Produce and Characterize Periarticular Heterotopic Ossification of the Hip
Abstract
Heterotopic ossification (HO) is a pathologic process characterized by the formation of bone tissue in extraskeletal locations. The hip is a common location of HO, especially as a complication of arthroplasty. Here, we devise a first-of-its-kind mouse model of post-surgical hip HO and validate expected cell sources of HO using several HO progenitor cell reporter lines. To induce HO, an anterolateral surgical approach to the hip was used, followed by disclocation and acetabular reaming. Animals were analyzed with high-resolution roentgenograms and micro-computed tomography, conventional histology, immunohistochemistry, and assessments of fluorescent reporter activity. All the treated animals’ developed periarticular HO with an anatomical distribution similar to human patients after arthroplasty. Heterotopic bone was found in periosteal, inter/intramuscular, and intracapsular locations. Further, the use of either PDGFRα or scleraxis (Scx) reporter mice demonstrated that both cell types gave rise to periarticular HO in this model. In summary, acetabular reaming reproducibly induces periarticular HO in the mouse reproducing human disease, and with defined mesenchymal cellular contributors similar to other experimental HO models. This protocol may be used in the future for further detailing of the cellular and molecular mediators of post-surgical HO, as well as the screening of new therapies.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jun 27, 2022
- Source ID
- 10.1093/stcltm/szac042
Entities
People
- Aaron W James
- Adam Levin
- Benjamin Lévi
- Bradley Presson
- Edward Mccarthy
- Ginny Ching‐Yun Hsu
- Jiajia Xu
- Masnsen Cherief
- Qizhi Qin
- Robert Joel Tower
- Seungyong Lee
- Stefano Negri
- Yiyun Wang
- Zhao Li
Organizations
- American Cancer Society
- American College of Surgeons
- Johns Hopkins University
- National Institute of Arthritis and Musculoskeletal and Skin Diseases
- National Institute of Dental and Craniofacial Research
- National Institutes of Health
- United States Department of Defense
- University of Texas Southwestern Medical Center
- University of Verona