DNA Binding Kinetics of CTCF in vitro
Abstract
CTCF is a ubiquitously expressed transcription factor whose architectural role as an insulator is tied to its ability to form genomic loops and delimit boundaries of topologically associated domains (TADs). This capacity for binding of a single protein to affect distal regulation over megabases is both remarkable and poorly understood mechanistically. In current models, a cohesin‐associated complex expands a loop by tracking along DNA in both directions, while CTCF defines the loop size and position by halting this complex. However, a recent single molecule imaging study finds the DNA‐association lifetime of CTCF to be ten‐fold shorter than that of cohesin in vivo, suggesting that CTCF and cohesin act independently and dynamically (Hansen et al., 2017). This raises key questions about how CTCF forms and maintains domain boundaries and loops, and highlights the need for a careful study of CTCF‐DNA binding kinetics.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Apr 01, 2018
- Source ID
- 10.1096/fasebj.2018.32.1_supplement.523.6
Entities
People
- Adrian L. Sanborn
- Roger D. Kornberg
Organizations
- National Institutes of Health
- Stanford University