Heart Metabolic Responses to Exertional Heat Stroke Are Dependent Upon Sex
Abstract
In virtually all eukaryotic organisms, sex is an important variable affecting many physiological functions. In exertional heat stroke (EHS), the cardiac muscle plays a dual role to pump blood to the active skeletal muscles and to the periphery for heat exchange, but whether the metabolic responses occurring in the cardiac muscle after EHS are sex‐dependent remains unknown. In our pre‐clinical model of EHS, female mice run longer in the heat and this advantage seems to be regardless of body size. Our aim was to determine sex differences in cardiac muscle's metabolic biomarkers in response to a single session of EHS. Sixteen male and 16 female C57BL6J mice were trained to run on an incremental forced running wheel and were exposed to 37.5°C/~40% relative humidity while running to induce EHS. Hearts were harvested 3 h after the EHS trial. Control mice ran for the same relative duration and were sacrificed 4 days after the trial. Metabolites were extracted from the heart and analyzed by mass spectrometry. Metabolites were matched to a library of standards for identification and quantification. Time to achieve EHS was 121.16 ± 22 min for males and 189.25 ± 35 for females (pThe opinions or assertions contained herein are the private views of the author(s) and are not to be construed as official or reflecting the views of the Army or the DoD.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Apr 01, 2018
- Source ID
- 10.1096/fasebj.2018.32.1_supplement.590.10
Entities
People
- Alex J. Mattingly
- Brian Ingram
- Christian K. Garcia
- Danielle L. Ippolito
- Gerard P. Robinson
- Lisa R. Leon
- Orlando Laitano
- Thomas L Clanton
Organizations
- United States Army Research Institute of Environmental Medicine
- United States Department of Defense
- University of Florida