Effects of the Organophosphate Pesticide Chlorpyrifos on Muscle Architecture in 72 hour Zebrafish Embryos
Abstract
Chlorpyrifos (CPF) and other organophosphate pesticides (OP) are commonly used in the agricultural industries of the United States and developing countries. High concentrations of OP's in rivers and lakes have led to concerns for its negative effects on the wildlife and humans. These substances have been documented to act as neurotoxins that lead to the buildup of acetylcholinesterase in cholinergic neurons. This study was performed to determine the effects of CPF on the development of muscles for zebrafish embryos 72 hours post‐fertilization (hpf) in a calcium‐depleted medium. Using confocal microscopy of wholemount MF20 immuno‐labeled embryos, several morphological changes were observed. There was an increase in the length of muscle myomeres and width of individual muscle fibers in CPF exposed embryos. Additionally, some embryos showed strong muscle fiber disorganization. These observations show that CPF has a clear effect to muscle development and architecture possibly due to increased cholinergic stimulation during development.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Apr 01, 2018
- Source ID
- 10.1096/fasebj.2018.32.1_supplement.644.18
Entities
People
- Erica A. Fradinger
- Robert C. Willrich
- Taylor H. Watanabe
Organizations
- Army Research Office
- United States Department of Defense
- Whittier College