Designed Metal‐Mediated Protein Dimerization
Abstract
The specificity and stability of protein/protein interactions is of vital interest to the pharmaceutical and biotechnological industries. Incorporation of metal‐binding sites at target protein interfaces may be one approach to improve the specificity and affinity of naturally occurring and designed protein‐protein interactions. The main goal for this research was to generate a metal‐mediated protein interface and study the effects of interfacial modification on protein‐protein affinity. Our approach recently resulted in the creation of a high‐affinity, zinc‐mediated protein homodimer.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Apr 01, 2018
- Source ID
- 10.1096/fasebj.2018.32.1_supplement.798.6
Entities
People
- Brian J. Maniaci
- John J Love
Organizations
- San Diego State University
- United States Department of Defense