The Role of the Vacuolar (H+)‐ATPase in Neuroblastoma Cell Differentiation induced by microRNA 506‐3p
Abstract
Neuroblastoma is one leading causes of cancer‐related childhood deaths. Neuroblastoma arises from the developing sympathetic nervous system where neural crest cells fail to complete the differentiation process. The failure in the differentiation process initiated the investigation into differentiation‐inducing agents. MicroRNA 506‐3p (miRNA‐506) was previously identified to be a potent tumor suppressor and induced neuroblastoma cell differentiation. A gene expression array determined that the ATP‐driven proton pump, vacuolar (H+)‐ATPase (V‐ATPase) is directly targeted by the miRNA‐506. However, the role of V‐ATPase in neuroblastoma has not been investigated. We recently found that by using siRNAs to knock down V‐ATPase expression we were able to reduce neuroblastoma proliferation and cell viability. These results warrant further investigation to determine the molecular mechanisms through which V‐ATPase helps mediate the effects of miRNA‐506 on neuroblastoma cells.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Apr 01, 2018
- Source ID
- 10.1096/fasebj.2018.32.1_supplement.804.3
Entities
People
- Geraldo Medrano
- Liqin Du
- Zhenze Zhao
Organizations
- Texas State University
- United States Department of Defense