Therapeutic Exosome Preparations: Relative Bioactivities of Intra‐ and Extra‐Vesicular Components
Abstract
Adult progenitor cells from the heart known as cardiosphere derived cells (CDCs) are therapeutic candidates in active clinical development for conditions including heart failure, pulmonary hypertension and Duchenne muscular dystrophy. The mechanism of action of CDCs is paracrine, mediated principally by the secretion of extracellular vesicles (EVs) known as exosomes. Progenitor cell EVs represent an exciting therapeutic opportunity, but the ideal culture conditions and isolation methods are still under investigation. The objective of this study was to examine the effect of various conventional EV isolation methods upon signaling, uptake, and viability in human vein endothelial cells (HUVECs), cardiac fibroblasts, and neonatal rat cardiomyocytes (NRVMs). Specifically, we sought to distinguish effects attributable to the EVs themselves from those of external factors co‐isolated with, or adherent to, the EVs.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Apr 01, 2018
- Source ID
- 10.1096/fasebj.2018.32.1_supplement.840.8
Entities
People
- Eduardo Marban
- Jane Liu
- Kristin Luther
Organizations
- Heart Institute
- United States Department of Defense