Gene Profiles of Dengue Virus Targeted Host Biological Processes and Pathologic Markers
Abstract
Dengue is one of the most prevalent arthropod‐borne viral illnesses in humans with neither selective therapy nor an approved vaccine. Understanding its molecular targets and biological processes may contribute towards developing sensitive diagnostic markers and/or effective therapeutics. Leucocytes obtained from 15 US Army Ranger Cadets before and after 9 weeks training were in vitro treated with dengue virus serotype 2. cDNAs of isolated RNAs were profiled using glass microarray chips containing ∼10,000 human gene targets. Statistical analyses gave ∼180 genes specifically regulated by dengue both in the presence and absence of severe physiological stress. Ontological‐enrichment showed that genes forming spliceosome, ribosome, translation initiation and proteosome complexes were up regulated while those involved in translation elongation, protein modification and targeting were down regulated. It seems that dengue virus mainly targets host protein biosynthesis with the net effect of blocking formation of functional host proteins while it gears the host's machineries towards promoting its own replication and pathogenesis. Subsets of 180 genes were also specific to dengue even in the presence of infections with other pathogens. These have the potential to be used as diagnostic markers irrespective of the physiological and pathologic conditions of the host.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Mar 01, 2008
- Source ID
- 10.1096/fasebj.22.1_supplement.1030.3
Entities
People
- David Y. Yang
- Marti Jett
- Rasha Hammamieh
- Seid Muhie
Organizations
- Defense Threat Reduction Agency
- Georgetown University
- Walter Reed Army Institute of Research