A high protein diet maintains glucose production and enhances gluconeogenesis during exercise induced energy deficit

Abstract

Glucose production (GP) decreases when energy expenditure (EE) exceeds energy intake (EI), whether dietary macronutrient content modulates this response is unclear. We determined if increasing dietary protein (PRO) mitigates down‐regulation of glucose production (GP), glycogen breakdown (GB) and gluconeogenesis (GNG) during exercise‐induced energy deficit. Thirteen men (22 ± 1 yrs, VO2peak 60 ± 2 ml·kg−1·min‐1) increased EE 1000 kcal·d‐1 for 7‐d (D1‐7), following 4‐d baseline (BL1‐4) of normal EE (i.e. pre‐study). Volunteers consumed BL EI all 11‐d, producing 1,000 kcal d−1energy deficit on D1‐7. Subjects ate a 55% carbohydrate diet with either 0.9 (DEF, n=7) or 1.8 (DEF‐HP, n=6) g pro kg−1 d−1. GP and GNG were measured in the fasted, rested state on BL4, D3 and D8 using 4‐h primed, continuous infusions of [6,6‐2H2]glucose (dilution method) and [2‐13C]glycerol (MIDA technique), respectively. DEF experienced 17% decline in GP (2.3 ± 0.3 mg·kg−1·min−1), due to 24% decline in GB (1.4 ± 0.1 mg·kg−1·min−1) with unchanged GNG compared to BL4. Conversely, DEF‐HP maintained GP, attributed to 7% increase in GNG (1.0 ± 0.1 mg·kg−1·min−1), but no change in GB compared to BL4. Rate of GP for DEF‐HP was higher than DEF on D8 (1.1 ± 0.1 mg·kg−1·min−1 and 0.9 ± 0.0 mg·kg−1·min−1, respectively). Additional dietary protein mitigated effects of exercise‐induced energy deficit on GP. Research funded by MRMC.

Document Details

Document Type
Pub Defense Publication
Publication Date
Mar 01, 2008
Source ID
10.1096/fasebj.22.1_supplement.1088.1

Entities

People

  • Carmen Castaneda‐sceppa
  • Ellen Glickman
  • Jean‐marc Schwartz
  • Jennifer C Rood
  • Matthew A. Pikosky
  • Tracey J. Smith

Organizations

  • Pennington Biomedical Research Center
  • Tufts University
  • United States Army Medical Research and Development Command

Tags

Fields of Study

  • Biology
  • Medicine

Readers

  • Analytical Mechanics
  • Exercise and Sports Science.
  • Traumatic Brain Injury (TBI) and Cognitive Aging in the Guam and Border Populations Affected by Alzheimer's Disease and Tau-Associated Dementias.

Technology Areas

  • Space