EFFECTS OF ISOVOLEMIC HEMODILUTION ON TISSUE OXYGEN CONSUMPTION USING A HEMOGLOBIN‐BASED OXYGEN CARRIER AND HUMAN SERUM ALBUMIN
Abstract
This microcirculatory study compared the effects on oxygen transport of two hemodilution fluids: HBOC‐201 (Biopure Corp., Cambridge, MA) a Hemoglobin‐Based Oxygen Carrier (HBOC), and 5.9% Human Serum Albumin (HSA) an iso‐oncotic non‐oxygen carrying colloid solution. Measurements using intravital microscopy were made on the spinotrapezius muscle of Sprague‐Dawley rats. Interstitial PO2 was measured using phosphorescence quenching microscopy, and recorded before and after isovolemic hemodilutions (HD) at hematocrits of 40% (baseline), 30% (moderate HD) and 15% (severe HD). Oxygen consumption (VO2) of the spinotrapezius muscle was derived from PO2 recordings following the rapid inflation of a Saran bag placed around the objective. When the bag was inflated, blood flow in the muscle was arrested and PO2 rapidly fell over several seconds; the rate of decline of PO2 was proportional to VO2. For moderate HD with HBOC‐201 interstitial PO2 did not change from baseline conditions (Hct~40%), while HD with HSA showed a decrease. For severe HD (Hct~15%) both PO2 and VO2 were significantly lower for the HSA group than for the HBOC‐201 group. These findings indicate that HBOC‐201 maintains both a higher PO2 and VO2 during hemodiluted states compared with a non‐oxygen carrying colloid solution (HSA).
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Mar 01, 2008
- Source ID
- 10.1096/fasebj.22.1_supplement.1141.24
Entities
People
- Bjorn Kyungsuck Song
- Roland N. Pittman
Organizations
- National Institutes of Health
- Naval Medical Research Center
- United States Department of Defense
- Virginia Commonwealth University