Dual lumen catheter for heparin‐free hemorrhage in rats: design, construction, and use
Abstract
Intermittent blood withdrawal is required for most studies of hemorrhagic shock; however, these experiments are often complicated by clot formation within the withdrawal catheter. In order to avoid the systemic administration of heparin and we sought to construct a dual lumen catheter that would allow for regional anticoagulation within the withdrawal catheter. The free end of the outer catheter was connected to a computer controlled withdrawal pump while the inner catheter was connected to a similarly controlled infusion pump. Whenever blood was withdrawn through the outer catheter, citrate was infused into the inner catheter at fixed volume ratio of 1:9 infusion:withdrawal. In rodent hemorrhage experiments, the dual lumen catheter was able to successfully bleed the animal to hemodynamic decompensation (avg. volume = 9.5 ± 2.9 ml) without clotting. The ionized calcium of the rats did not change beyond that which would be normally expected from severe hemorrhage. (1.21 ±0.07 mM control vs 1.08 ± 0.18 after hemorrhage). Prothrombin times were not altered during hemorrhage. We conclude that a dual‐lumen catheter using regional citrate anticoagulation can be used as a means of achieving heparin‐free hemorrhage of rodents. The design of the catheter prevents systemic effects of the citrate on the animal and could potentially be used with other anticoagulants. Funded by US Army Medical Research and Materiel Command.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Mar 01, 2008
- Source ID
- 10.1096/fasebj.22.1_supplement.1227.13
Entities
People
- Chae‐hyuk Lee
- Elizabeth Barrows
- James Atkins
- James Oliver
- Peter Quinn
- Raoul Gonzales
Organizations
- United States Army Medical Research and Development Command