Engineering an in vitro environment to study regeneration
Abstract
During limb regeneration in the newt, skeletal muscle provides a reservoir for uncommitted stem‐like blastema cells that participate in the replacement of the lost structure. However, it is not clear to what extent the activation of muscle‐associated satellite cells and/or fragmentation of multinucleate myofibers contribute to the blastema. During blastema formation, the composition of the extracellular matrix (ECM) undergoes dramatic changes that may provide cues for cell dedifferentiation. Using time‐lapse microscopy on in vitro cultures, we have shown that hyaluronic acid induces dedifferentiation and fragmentation of myotubes whereas fibronectin or matrigel leads to myotube formation.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Mar 01, 2008
- Source ID
- 10.1096/fasebj.22.1_supplement.580.1
Entities
Organizations
- Defense Advanced Research Projects Agency
- National Institutes of Health