Mustard Gas‐Induced Alteration of Retinoid Metabolism/Signaling in Guinea Pig Lung
Abstract
Mustard gas exposure causes inflammatory lung diseases, including Adult Respiratory Distress Syndrome (ARDS). Retinoids (retinoic acid, retinol, retinyl palmitate) are important for growth and differentiation of various cell types. Retinoids are known for their role in regulation of gene expression and as effective inhibitors of chemical carcinogenesis in epithelial tumors such as those of the skin, lung, breast etc. Various environmental toxicants are known to alter the delicate metabolic balance of these compounds in the body. HPLC method has been used for analysis of retinoids in a single run in guinea pig lung after exposure of CEES (2‐chloroethyl ethyl sulfide), a mustard analogue. Adult guinea pigs were intratracheally injected with CEES in ethanol. Control animals were injected with only ethanol. Animals were sacrificed and lungs removed after perfusion with physiological saline. Lung injury was established by measuring leakage of iodinated‐BSA into lung tissue. HPLC analysis of lungs showed that there was an increase in retinoic acid (RA) and a decrease in retinol and retinol esters due to CEES exposure. Expression of RA receptors (α, ß) and cellular retinoic acid binding protein was also increased. Further studies are being carried out to elucidate the functional consequence of the early increase in the level of RA by CEES exposure (Supported by US Army Grant W81XWH‐06‐2‐0044)
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Mar 01, 2008
- Source ID
- 10.1096/fasebj.22.1_supplement.646.4
Entities
People
- Michael W Schäffer
- Salil K. Das
- Shyamali Mukherjee
- Somdutta Sinha Roy
Organizations
- Meharry Medical College
- United States Army