Assessing angiogenic activity of cells in the presence of oxygen generating biomaterials
Abstract
Upon implantation the center of a large (>1 cm3) cell seeded tissue scaffold typically becomes hypoxic and eventually necrotic. This is primarily due to the diffusion limitation of oxygen. We have developed a novel class of biomaterials capable of in situ sustained release of oxygen. Such materials would help improve cell viability within tissue scaffolds while the tissue becomes integrated with the host. However, angiogenesis is an oxygen dependent process. When hypoxia occurs, cells produce angiogenic factors, such as VGEF, to stimulate blood vessel growth to raise the oxygen tensions within the cellular environment. One concern in using materials capable of generating of oxygen is that it will hinder angiogenesis leading to the ultimate failure of the implant tissue. We examined the effect of oxygen generating materials on angiogenesis using an in vitro angiogenesis assays on HUVECs and have found that it does not suppress angiogenesis. Our results suggest that oxygen can be generated as a sufficient level that it can increase cell viability without suppressing angiogenesis. This was supported by grants from the NIH and DOD.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Apr 01, 2009
- Source ID
- 10.1096/fasebj.23.1_supplement.951.8
Entities
People
- Benjamin S. Harrison
- Catherine L. Ward
- Evelyn Eisele
- Nathan Haines
- Ravi K Patel
Organizations
- National Institutes of Health
- United States Department of Defense
- Wake Forest University