Mass‐spectrometric analysis of changes in brain lipid expression and distribution after brain injury
Abstract
Mechanical impact injuries to the cortex of rats induce neuronal loss, vascular damage, glial activation, infiltration of circulating immune cells, and edema, followed by partial revascularization, partial neuronal recovery and remodeling of neuronal connectivity. We report here changes in lipid metabolism in the region of injury after a controlled cortical impact (CCI) injury to the cortex of anesthetized Sprague‐Dawley rats. At selected time points after induction of a CCI injury inducing tissue damage and subsequent loss of some cortical tissue but not major functional impairment, brains were removed and processed for imaging mass spectrometry of 16 μm coronal sections through the region of injury. Following injury, significant time‐related changes in numerous lipid species in both gray and white matter around and through the site of injury were observed. Specifically, increases and decreases in the levels of specific sphingomyelin species were noted at 24 hrs, 3 days and 7 days after CCI injury. Phosphatidylcholine 36a:2 was found in greater abundance in both grey and white matter in the area of injury than in the contralateral cortex, while another phosphatidylcholine 38:4 showed no change in distribution between sham and injured animals. Lipid imaging presents new insights into the cellular consequences of injury, (Supported by US Dept of Defense through the CNRM).
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Apr 01, 2012
- Source ID
- 10.1096/fasebj.26.1_supplement.1046.4
Entities
People
- A.S. Woods
- Brian M Cox
- Gregory Bull
- Jeremy Post
- Shawn Gouty
Organizations
- Center for Neuroscience and Regenerative Medicine
- Uniformed Services University of the Health Sciences