Decreased tight junction gene expression in the duodenum following heat stroke in F344 rats
Abstract
Heat stroke (HS) morbidity and mortality is initiated by endotoxin escape (EE) from the intestinal tight junction barrier (TJB) although in vivo the molecular mechanisms of EE are not well understood. The current study tested the hypothesis that in the duodenum of male Fischer 344 (F344) rats tight junction gene expression is decreased following HS. F344 rats (276.6 gms) were heated until a maximum core temperature (Tc,Max; radiotelemetry ±0.1°C) of 41.8°C and allowed to recover for one, two or ten days. Duodenum gene expression of tight junction protein 1 (−2.06 fold), occludin (−2.13 fold), claudin 2 (−3.86 fold) and claudin 3 (−2.22 fold) was significantly decreased at Tc,Max (n=7; p<0.05) versus non‐heated controls (n=7). Claudin 2 gene expression was the only TJB protein that remained significantly decreased at one day (n=7;−2.93 fold, p<0.05) when compared to controls (n=8; p<0.05). At two and ten days gene expression of tight junction proteins was not different from control animals. These data suggest that decreased expression of TJB proteins may facilitate EE during the first 48h following HS. Research supported by MRMC. Author views not official US Army or DoD policy.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Apr 01, 2012
- Source ID
- 10.1096/fasebj.26.1_supplement.1084.15
Entities
People
- Bryan G. Helwig
- Jermaine A. Ward
- Lisa R. Leon
Organizations
- United States Army Medical Research and Development Command