Divergent intramuscular mTORC1 signaling responses to habitual consumption of milk‐and soy‐based energy restricted diets
Abstract
The effects of milk‐or soy‐based, high‐protein, energy restricted (ER) diets on intracellular regulators of muscle mass are not well described. Eighty male Sprague‐Dawley rats were randomly divided into groups and fed energy adequate (EA) and ER (60% of average ad libitum EA feed intake) diets containing two protein sources (milk protein concentrate: MPC; or isoflavone‐free soy isolate: SOY) and levels (control protein: CP, 10%; high protein: HP, 35%) for 16 weeks. Basal protein expression and phosphorylation of mammalian target of rapamycin complex 1 (mTORC1) associated proteins were assessed using multiplex. Phosphorylation of mTOR was lower (P S6K1 expression was lower (P P P P S6K1 phosphorylation was higher (P < 0.05) for SOY versus MPC, regardless of energy status and protein level. These data demonstrate divergent mTORC1 responses to soy‐and milk‐based energy restricted diets but suggest that habitual consumption of soy‐based diets may enhance protein synthetic responses to feeding by upregulating the expression of key mTORC1 signaling proteins. Supported by Dairy Research Institute and USAMRMC
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Apr 01, 2013
- Source ID
- 10.1096/fasebj.27.1_supplement.631.9
Entities
People
- Andrew J Young
- Charles R Wulff
- Erin Gaffney‐stomberg
- Gregory G Lin
- James P. Mcclung
- Nancy E. Murphy
- Stefan M. Pasiakos
Organizations
- United States Army Medical Research and Development Command