Effects of Resuscitation Fluids following Hemorrhagic Shock on Venular Glycocalyx in Rats
Abstract
The protection mechanisms of resuscitative agents after hemorrhagic shock (HS) are still unclear. For instance, fresh frozen plasma (FFP) may exert its positive effects by replacing lost coagulation factors and by acting upon the endothelium. We compared different resuscitation fluids in their ability to restore the structural integrity of anesthetized rat cremaster venular endothelial glycocalyx (EG) in vivo, as estimated by its thickness using intravital microscopy and fluorescent dextrans. After 1 h of HS (bleeding target: 40% of total blood volume), rats were randomized into 4 groups (6–8 rats/group): HEM‐ no resuscitation; LR– lactated Ringer's; HEX–Hextend and FFP. Baseline EG averaged 0.425 ± 0.015 μm (n=121). After resuscitation, EG was reduced by nearly 70% in HEM, LR and HEX groups to an average 0.132 ± 0.010 μm. However, in rats that received FFP, EG recovered to a thickness of 0.532 ± 0.060 μm, not significantly different from baseline. Syndecan‐1 plasma levels also rose significantly in all groups except in rats that received FFP, where levels returned to baseline. All groups showed significant reductions in venular blood flow, but only HEX rats showed full recovery of flow. The data suggest that plasma but neither colloid nor crystalloid resuscitation support vascular stabilization by reconstitution of EG after HS‐mediated degradation.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Apr 01, 2013
- Source ID
- 10.1096/fasebj.27.1_supplement.900.11
Entities
People
- Ivo P. Torres Filho
- Jill Sondeen
- Lisa Ji
- Luciana Torres
- Michael Dubick
Organizations
- United States Army Institute of Surgical Research
- United States Army Medical Research and Development Command