Antioxidant activity of currently available resuscitation fluids in vitro (1157.5)
Abstract
Addition of antioxidants to resuscitation from hemorrhage reportedly improves outcomes in experimental animals. The present study investigated whether currently available resuscitation fluids have intrinsic antioxidant activity. Direct antioxidant activity of normal saline, hypertonic saline alone or with Dextran, Ringer’s solution alone or with lactate, pyruvate, or β‐hydroxybutyrate, Hextend, Voluven, plasma, 5% albumin and Hyvisc drag‐reducing compounds were investigated in duplicate in systems to either inhibit xanthine oxidase (XO) or superoxide dismutase (SOD) generated oxidant production or measure antioxidant status. N‐acetylcysteine (NAC) was included as a positive control. In the XO system, Hextend showed minor antioxidant activity, whereas Hyvisc preparations, plasma and Ringer’s showed antioxidant activity similar to NAC. All fluids tested inhibited superoxide production in the SOD system about 10% compared to over 30% by NAC. There did not appear to be any significant difference between colloids and crystalloids or compounds such as pyruvate or albumin at the concentrations selected. Taken together these data suggest that currently available resuscitation fluids have minor, if any, intrinsic antioxidant activity. Reported antioxidant properties of these compounds in vivo, most likely reflects indirect activity through their interactions with biological systems.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Apr 01, 2014
- Source ID
- 10.1096/fasebj.28.1_supplement.1157.5
Entities
People
- Dana Grubbs
- David R Jacobson
- Johnny Barr
- Michael Dubick
Organizations
- United States Army Institute of Surgical Research
- United States Army Medical Research and Development Command