Lipid‐dependent proliferation of breast and pancreatic cancer cell lines (605.7)
Abstract
Cancer cells rely heavily on anaerobic glycolysis for energy production, and have the capacity to synthesize their own sup¬ply of biologically active compounds of predominantly lipid origin. We hypothesize that any perturbation of this lipid pool, caused by drug treatment or changes in lipid metabolism, will result in significant alterations in the biological processes of these cells and result in cell death. In these studies, the lipid content of breast and pancreatic cell culture models has been assessed. Staining with Nile Red and Oil Red O indicate that primary cancer cell lines contain significantly higher lipid levels than metastatic lines and that the subcellular localization of these lipids also varies. Separation, identification, and quantitation of total lipid extracts by TLC and LCMS indicates that the proportions of phospholipids also vary in relation to the level of tumor cell differentiation. Treatment with drugs and transfection of cells with plasmids for specific UGT isoforms, which are known to conjugate various bioactive lipids and to be down regulated in several cancers, affects the lipid content and results in decreased cell proliferation and death. Nanodelivery of these drugs and plasmids significantly enhances these effects. Examining the role of lipids in cancer cell homeostasis is a novel approach to cancer research.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Apr 01, 2014
- Source ID
- 10.1096/fasebj.28.1_supplement.605.7
Entities
People
- Alex Biris
- Anna Radominska‐pandya
- Lamya Saeed
- Sebastian Pyrek
- Tariq Fahmi
Organizations
- United States Department of Defense
- University of Arkansas at Little Rock