Ubiquinol reduces leukocyte‐endothelium interactions in the mesenteric microcirculation following hemorrhagic shock and fluid resuscitation (667.1)
Abstract
Hemorrhagic shock (HS) and fluid resuscitation (FR) generate reactive oxygen species (ROS) which is a major factor in post‐hemorrhage inflammation and tissue injury. The hypoxia created by HS/reperfusion injury (RI) alters the microvascular blood flow and microvascular oxygen. The objective of the study was to determine if ubiquinol (reduced form of coenzyme Q10) reduces leukocyte‐endothelium interaction following HS/FR. Intravital microscopy was used to measure leukocyte‐endothelium interactions in the rat mesentery following 1‐hour HS and 2‐hour post FR with or without ubiquinol. HS was induced by removing ~40% of the rats’ blood volume resulting in a mean arterial blood pressure (MABP) being ~44 mmHg for 1‐hour. Immediately afterwards ubiquinol (1 mg/100 g body weight) was infused intra‐arterially. RI was produced by replacing the shed blood and Lactate Ringer’s. Below are the findings (n=6, *p < 0.05):
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Apr 01, 2014
- Source ID
- 10.1096/fasebj.28.1_supplement.667.1
Entities
People
- Amanda Thimmesch
- Janet Pierce
- John Wood
- Naomi Holloway
- Qiuhua Shen
- Richard J. Clancy
Organizations
- Uniformed Services University of the Health Sciences
- United States Department of Defense
- University of Kansas