Genetic and environmental factors that regulate tandem repeat variation in coding regions
Abstract
More than 10% of all proteins harbor a region of repetitive amino acid sequence. Instability within the DNA that codes for these sequences tunes protein function by varying the number of tandem repeats that are expressed and contributes to phenotypic diversity. Based on the repetitive C‐terminal domain of RNA polymerase II subunit Rpb1p, Our lab has developed a powerful reporter system for measuring expansion and contraction of tandem repeats in coding regions (Morrill et al., J. Biol. Chem, 2016). Here we use this system to identify the pathways involved in both repeat expansion and contraction in the budding yeast S. cerevisiae. We also uncovered a direct role between environmental stress and repeat variation and provide links to how TR variation may be contributing to organismal adaptation to stress.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Apr 01, 2017
- Source ID
- 10.1096/fasebj.31.1_supplement.591.4
Entities
People
- Stephen M. Fuchs
Organizations
- Army Research Office
- Tufts University