Nanoparticle‐based biosensors for the detection of lecithin:cholesterol acyltransferase activity
Abstract
Lecithin:cholesterol acyltransferase (LCAT) is an enzyme that catalyzes the esterification of free cholesterol carried in high‐density lipoproteins (HDL). Esterification of cholesterol results in internalization of cholesteryl ester (CE) within the core of maturing HDLs. Of the HDL species, increased levels of CE‐rich HDLs are inversely correlated with the development of cardiovascular disease. Prior research has established that the activity of LCAT is influenced by numerous external physiological stimuli. Our lab has previously developed nanoparticles (HDL‐NP) that mimic natural HDLs with regard to size, shape, surface chemistry, and cholesterol binding function. Herein, we describe the application of HDL‐NP as specific activators of LCAT and biosensors for assaying LCAT activity.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Apr 01, 2017
- Source ID
- 10.1096/fasebj.31.1_supplement.829.7
Entities
People
- Colby Shad Thaxton
- Dongho Kim
- Rohun U. Palekar
Organizations
- Air Force Research Laboratory
- Northwestern University