High‐throughput Gene Expression Profiling of Myeloid Cells in Mechanically Ventilated Patients with Pneumonia
Abstract
Transcriptomic analysis of peripheral blood leukocytes has improved our understanding of the host response to pneumonia. However, this approach is limited as these cells may not be directly exposed to causative pathogens in the alveolar space. Non‐bronchoscopic bronchoalveolar lavage (NBBAL) allows for rapid sampling of the distal airspaces in critically ill patients. We have adapted protocols for isolating individual myeloid cell populations from human lung tissue using fluorescence‐activated cell sorting (FACS) to isolate alveolar macrophages from NBBAL fluid in mechanically ventilated patients with suspected pneumonia.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Apr 01, 2017
- Source ID
- 10.1096/fasebj.31.1_supplement.908.21
Entities
People
- Alexander V. Misharin
- G.r. Scott Budinger
- James M. Walter
- Kishore Reddy Anekalla
- Monica Chi
- Paul A Reyfman
- Richard Wunderink
Organizations
- Northwestern University
- United States Department of Defense