Identification of proteins in the adhesive trails of the diatom Amphora coffeaeformis
Abstract
Throughout all kingdoms of life, a large number of adhesive biomolecules have evolved to allow organisms to adhere to surfaces underwater. Proteins play an important role in the adhesion of numerous marine invertebrates (e.g. mussels, sea stars, sea urchins) whereas much less is known about the biological adhesives from marine plants, including the diatoms. Diatoms are unicellular microalgae that together with bacteria dominate marine biofilms in sunlit habitats. In this study we present the first proteomics analyses of the diatom adhesive material isolated from the tenacious fouling species Amphora coffeaeformis . We identified 21 proteins, of which 13 are diatom-specific. Ten of these proteins share a conserved C-terminal domain, termed GDPH domain, which is widespread yet not ubiquitously present in all diatom classes. Immunofluorescence localization of a GDPH domain bearing protein (Ac629) as well as two other proteins identified in this study (Ac1442, Ac9617) demonstrated that these are components of the adhesive trails that are secreted by cells that glide on surfaces.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Sep 09, 2019
- Source ID
- 10.1098/rstb.2019.0196
Entities
People
- Jennifer Klemm
- Martina Lachnit
- Matthias T Buhmann
- Nicole Poulsen
- Nils Kroeger
Organizations
- Air Force Office of Scientific Research
- German Research Foundation
- Technische Universität Dresden