A panel of diverse Klebsiella pneumoniae clinical isolates for research and development
Abstract
Klebsiella pneumoniae are a leading cause of healthcare-associated infections worldwide. In particular, strains expressing extended-spectrum β-lactamases (ESBLs) and carbapenemases pose serious treatment challenges, leading the World Health Organization (WHO) to designate ESBL and carbapenem-resistant Enterobacteriaceae as ‘critical’ threats to human health. Research efforts to combat these pathogens can be supported by accessibility to diverse and clinically relevant isolates for testing novel therapeutics. Here, we describe a panel of 100 diverse K. pneumoniae isolates that are publicly available to assist the research community in this endeavour. Whole-genome sequencing (WGS) was performed on 3878 K . pneumoniae clinical isolates housed at the Multidrug-Resistant Organism Repository and Surveillance Network. The isolates were cultured from 63 facilities in 19 countries between 2001 and 2020. Core-genome multilocus sequence typing and high-resolution single-nucleotide polymorphism-based phylogenetic analyses captured the genetic diversity of the collection and were used to select the final panel of 100 isolates. In addition to known multidrug-resistant (MDR) pandemic lineages, the final panel includes hypervirulent lineages and isolates with specific and diverse resistance genes and virulence biomarkers. A broad range of antibiotic susceptibilities, ranging from pan-sensitive to extensively drug-resistant isolates, are described. The panel collection, and all associated metadata and genome sequences, are available at no additional cost and will be an important resource for the research community and for the design and development of novel antimicrobial agents and diagnostics against this important pathogen.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- May 04, 2023
- Source ID
- 10.1099/mgen.0.000967
Entities
People
- Ana C. Ong
- Anthony R. Jones
- Emil P. Lesho
- François Lebreton
- Jason W. Bennett
- Joshua A. Rosado-mendez
- Katharine F. Lane
- Lan N. Preston
- Mary Hinkle
- Melissa J. Martin
- Michael Julius
- Paige E. Waterman
- Patrick Mc Gann
- Rosslyn Maybank
- William Stribling
- Yoon I. Kwak
Organizations
- Armed Forces Research Institute of Medical Sciences
- Rochester General Hospital
- Uniformed Services University of the Health Sciences
- United States Army Medical Research and Development Command
- Walter Reed Army Institute of Research