Nutrient-dependent morphological variability of Bacteroides thetaiotaomicron

Abstract

Unique morphologies can enable bacteria to survive in their native environment. Furthermore, many bacteria change their cell shape to adapt to different environmental conditions. For instance, some bacteria increase their surface area under carbon or nitrogen starvation. Bacteriodes thetaiotaomicron is an abundant human gut species; it efficiently degrades a number of carbohydrates and also supports the growth of other bacteria by breaking down complex polysaccharides. The gut provides a variable environment as nutrient availability is subject to the diet and health of the host, yet how gut bacteria adapt and change their morphologies under different nutrient conditions has not been studied. Here, for the first time, we report an elongated B. thetaiotaomicron morphology under sugar-limited conditions using live-cell imaging; this elongated morphology is enhanced in the presence of sodium bicarbonate. Similarly, we also observed that sodium bicarbonate produces an elongated-length phenotype in another Gram-negative gut bacterium, Escherichia coli . The increase in cell length might provide an adaptive advantage for cells to survive under nutrient-limited conditions.

Document Details

Document Type
Pub Defense Publication
Publication Date
Jul 01, 2020
Source ID
10.1099/mic.0.000924

Entities

People

  • Aathmaja Anandhi Rangarajan
  • Julie S. Biteen
  • Nicole M Koropatkin

Organizations

  • Army Research Office
  • University of Michigan

Tags

Fields of Study

  • Biology
  • Environmental science

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