Host kinase CSNK2 is a target for inhibition of pathogenic β-coronaviruses including SARS-CoV-2

Abstract

Inhibition of the protein kinase CSNK2 with any of 30 specific and selective inhibitors representing different chemotypes, blocked replication of pathogenic human and murine β-coronaviruses. The potency of in-cell CSNK2A target engagement across the set of inhibitors correlated with antiviral activity and genetic knockdown confirmed the essential role of the CSNK2 holoenzyme in β-coronavirus replication. Spike protein uptake was blocked by CSNK2A inhibition, indicating that antiviral activity was due in part to a suppression of viral entry. CSNK2A inhibition may be a viable target for development of new broad spectrum anti-β-coronavirus drugs.

Document Details

Document Type
Pub Defense Publication
Publication Date
Jan 04, 2022
Source ID
10.1101/2022.01.03.474779

Entities

People

  • Alison D Axtman
  • Armin Bayati
  • Boyd L Yount
  • Carrow Wells
  • Edcon Chang
  • Emily A Madden
  • Erik M. Lenarcic
  • Jason W. Brown
  • Jeffery L. Smith
  • Mark T Heise
  • Nathaniel J. Moorman
  • Peter S McPherson
  • Ralph S. Baric
  • Rebekah J. Dickmander
  • Sharon Taft-Benz
  • Timothy M Willson
  • Xuan Yang

Tags

Fields of Study

  • Biology

Readers

  • Infectious Disease/Epidemiology
  • Molecular Biology and Genetics
  • Molecular Genetics

Technology Areas

  • Biotechnology