Human Immune Cell Epigenomic Signatures in Response to Infectious Diseases and Chemical Exposures
Abstract
Variations in DNA methylation patterns in human tissues have been linked to various environmental exposures and infections. Here, we identified the DNA methylation signatures associated with multiple exposures in nine major immune cell types derived from peripheral blood mononuclear cells (PBMCs) at single-cell resolution. We performed methylome sequencing on 111,180 immune cells obtained from 112 individuals who were exposed to different viruses, bacteria, or chemicals. Our analysis revealed 790,662 differentially methylated regions (DMRs) associated with these exposures, which are mostly individual CpG sites. Additionally, we integrated methylation and ATAC-seq data from same samples and found strong correlations between the two modalities. However, the epigenomic remodeling in these two modalities are complementary. Finally, we identified the minimum set of DMRs that can predict exposures. Overall, our study provides the first comprehensive dataset of single immune cell methylation profiles, along with unique methylation biomarkers for various biological and chemical exposures.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jun 30, 2023
- Source ID
- 10.1101/2023.06.29.546792
Entities
People
- Andrew Aldridge
- Anna Bartlett
- Anna Maria Walley
- Anthony K. Smith
- Bei Wei
- Cesar Barragan
- Christopher W Woods
- Dana B. Barr
- Elizabeth A. Petzold
- Fabian Müller
- Felicia Ruffin
- Flora Castellino
- German Nudelman
- Haili Song
- Hanqing Liu
- Huaming Chen
- Irem B. Gündüz
- Irene Ramos
- Jennifer L. Beare
- Jingtian Zhou
- Jordan Altshul
- Joseph Nery
- Joseph R. Ecker
- Lisa L. Satterwhite
- Manoj Hariharan
- Mia Kenworthy
- Micah T McClain
- Parinya Panuwet
- Rachel R. Spurbeck
- Rosa Castanon
- Sindhu Vangeti
- Stuart C. Sealfon
- Thomas Evans
- Thomas W. Burke
- Timothy J. Broderick
- Todd Norell
- Vance G. Fowler Jr.
- Vince Rothenberg
- Wei Tian
- Wenliang Wang
- William J. Greenleaf
- Wubin Ding
- Xiling Shen