Deubiquitinase USP20 promotes breast cancer metastasis by stabilizing SNAI2

Abstract

SNAI2/SLUG, a metastasis-promoting transcription factor, is a labile protein that is degraded through the ubiquitin proteasome degradation system. Here, we conducted comprehensive gain- and loss-of-function screens using a human DUB cDNA library of 65 genes and an siRNA library of 98 genes, and identified USP20 as a deubiquitinase (DUB) that regulates SNAI2 ubiquitination and stability. Further investigation of USP20 demonstrated its function in promoting migration, invasion, and metastasis of breast cancer. USP20 positively correlates with SNAI2 protein level in breast tumor samples, and higher USP20 expression is associated with poor prognosis in ER− breast cancer patients.

Document Details

Document Type
Pub Defense Publication
Publication Date
Sep 17, 2020
Source ID
10.1101/gad.339804.120

Entities

People

  • Hanqiu Zheng
  • Minhong Shen
  • Ruina Zhang
  • Wenyang Li
  • Yi-zhou Jiang
  • Yibin Kang
  • Yong Wei
  • Zhi-ming Shao

Organizations

  • Brewster Place
  • Istituto Superiore di Sanità
  • United States Department of Defense

Tags

Readers

  • Breast cancer cell signaling and growth regulation.
  • Marine Mammal Biology
  • Molecular Biology and Genetics