Recounting the FANTOM CAGE-Associated Transcriptome
Abstract
Long noncoding RNAs (lncRNAs) have emerged as key coordinators of biological and cellular processes. Characterizing lncRNA expression across cells and tissues is key to understanding their role in determining phenotypes, including human diseases. We present here FC-R2, a comprehensive expression atlas across a broadly defined human transcriptome, inclusive of over 109,000 coding and noncoding genes, as described in the FANTOM CAGE-Associated Transcriptome (FANTOM-CAT) study. This atlas greatly extends the gene annotation used in the originalrecount2resource. We demonstrate the utility of the FC-R2 atlas by reproducing key findings from published large studies and by generating new results across normal and diseased human samples. In particular, we (a) identify tissue-specific transcription profiles for distinct classes of coding and noncoding genes, (b) perform differential expression analysis across thirteen cancer types, identifying novel noncoding genes potentially involved in tumor pathogenesis and progression, and (c) confirm the prognostic value for several enhancer lncRNAs expression in cancer. Our resource is instrumental for the systematic molecular characterization of lncRNA by the FANTOM6 Consortium. In conclusion, comprised of over 70,000 samples, the FC-R2 atlas will empower other researchers to investigate functions and biological roles of both known coding genes and novel lncRNAs.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Feb 20, 2020
- Source ID
- 10.1101/gr.254656.119
Entities
People
- Aleksey Stupnikov
- Alexander Favorov
- Andrew E Jaffe
- Benjamin Langmead
- Chi-wai Yip
- Christopher Wilks
- Chung-chau Hon
- Diego F Sanchez
- Eddie Luidy Imada
- Francisco P Lobo
- Glória Regina Franco
- Harukazu Suzuki
- Jay W. Shin
- Jeffrey T. Leek
- Kayoko Yasuzawa
- Leonardo Collado-Torres
- Luigi Marchionni
- Masayoshi Itoh
- Michiel Jl de Hoon
- Naoto Kondo
- Piero Carninci
- Takeya Kasukawa
- Tejasvi Matam
- Wikum Dinalankara
Organizations
- Congressionally Directed Medical Research Programs
- Fundação de Amparo à Pesquisa do Estado de Minas Gerais
- National Cancer Institute
- National Institute of General Medical Sciences
- National Science Foundation
- Russian Center for Science Information
- United States Department of Defense