Small-angle X-ray microdiffraction from fibrils embedded in tissue thin sections
Abstract
Small-angle X-ray scattering (SAXS) from fibrils embedded in a fixed, thin section of tissue includes contributions from the fibrils, the polymeric matrix surrounding the fibrils, other constituents of the tissue, and cross-terms due to the spatial correlation between fibrils and neighboring molecules. This complex mixture severely limits the amount of information that can be extracted from scattering studies. However, availability of micro- and nano-beams has made the measurement of scattering from very small volumes possible, which, in some cases, may be dominated by a single fibrillar constituent. In such cases, information about the predominant species may be accessible. Nevertheless, even in these cases, the correlations between the positions of fibrils and other constituents have a significant impact on the observed scattering. Here, strategies are proposed to extract partial information about fibril structure and tissue organization on the basis of SAXS from samples of this type. It is shown that the spatial correlation function of the fibril in the direction perpendicular to the fibril axis can be computed and contains information about the predominant fibril structure and the organization of the surrounding tissue matrix. This has significant advantages over approaches based on techniques developed for X-ray solution scattering. Examples of correlation calculations in different types of samples are given to demonstrate the information that can be obtained from these measurements.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Nov 21, 2022
- Source ID
- 10.1107/s1600576722009955
Entities
People
- Abdullah Al Bashit
- Lee Makowski
- Lin Yang
- Prakash Nepal
Organizations
- Brookhaven National Laboratory
- National Institute of General Medical Sciences
- National Institute on Aging
- Northeastern University
- United States Department of Energy