Estimating Individual Contributions to Complex DNA SNP Mixtures.,

Abstract

High‐throughput sequencing (HTS) of large panels of single nucleotide polymorphisms (SNPs) provides an alternative or complimentary approach to short tandem repeats (STRs) panels for the analysis of complex DNA mixture forensic samples. For STRs, methods to estimate individual contribution concentrations compare capillary electrophoresis peak heights, peak areas, or HTS allele read counts within a mixture. This article introduces three approaches (mean, median, and slope methods) for estimating individual DNA contributions to forensic mixtures for HTS/massively parallel sequencing (MPS) SNP panels. For SNPs, the major:minor allele ratios or counts, unique to each contributor, were compared to estimate contributor proportion within the mixture using the mean, median, and slope intercept for these alleles. The estimates for these three methods were typically within 5% of planned experimental contributions for defined mixtures.

Document Details

Document Type
Pub Defense Publication
Publication Date
Feb 22, 2019
Source ID
10.1111/1556-4029.14030

Entities

People

  • Darrell O Ricke
  • Eric Schwoebel
  • James J Watkins
  • Philip Fremont-Smith
  • Sara Stankiewicz
  • Tara Boettcher

Organizations

  • Massachusetts Institute of Technology
  • United States Air Force

Tags

Fields of Study

  • Biology

Readers

  • Analytical Chemistry
  • Molecular Genetics
  • Regression Analysis.