Long‐range PCR allows sequencing of mitochondrial genomes from environmental DNA
Abstract
As environmental DNA (eDNA) from macro‐organisms is often assumed to be highly degraded, current eDNA assays target small DNA fragments to estimate species richness by metabarcoding. A limitation of this approach is the inherent lack of unique species‐specific single‐nucleotide polymorphisms available for unequivocal species identification.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jul 14, 2017
- Source ID
- 10.1111/2041-210x.12836
Entities
People
- Brett P. Olds
- David M. Lodge
- Kristy Deiner
- Mark A. Renshaw
- Michael E. Pfrender
- Yiyuan Li
Organizations
- United States Department of Defense
- University of Notre Dame