Methodological standards for in vitro models of epilepsy and epileptic seizures. A TASK1‐WG4 report of the AES/ILAE Translational Task Force of the ILAE
Abstract
In vitro preparations are a powerful tool to explore the mechanisms and processes underlying epileptogenesis and ictogenesis. In this review, we critically review the numerous in vitro methodologies utilized in epilepsy research. We provide support for the inclusion of detailed descriptions of techniques, including often ignored parameters with unpredictable yet significant effects on study reproducibility and outcomes. In addition, we explore how recent developments in brain slice preparation relate to their use as models of epileptic activity.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Nov 01, 2017
- Source ID
- 10.1111/epi.13901
Entities
People
- Akio Ikeda
- Aristea S Galanopoulou
- Chou‐ching K. Lin
- Chris G Dulla
- Christophe Bernard
- Damir Janigro
- Howard P. Goodkin
- Joseph V Raimondo
- Marco De Curtis
- Premysl Jiruska
- Uwe Heinemann
Organizations
- Agence Nationale de la Recherche
- Albert Einstein College of Medicine
- Case Western Reserve University
- Charité
- Czech Science Foundation
- GSK
- Institute of Physiology
- Kyoto University
- Ministry of Health of the Czech Republic
- National Cheng Kung University
- National Institute of Neurological Disorders and Stroke
- Otsuka Pharmaceutical
- Tufts University
- United States Department of Defense
- University of Cape Town
- University of Virginia