Effect of a recombinant HIV gpl60 vaccine on monokine production
Abstract
An investigation was undertaken to determine whether a recombinant gp 160 envelope protein, which is currently being evaluated as a vaccine for AIDS, induces or modulates the production of tumour necrosis factor-alpha (TNF-α) or interleukin-1β (IL-1β). Incubation of monocytes from healthy, HIV-seronegative persons with 0.0001–1.0 μg of the recombinant vaccine did not result in the secretion of TNF-α or IL-1β, nor did the recombinant product augment or suppress monokine production by lipopolysaccharide (LPS) stimulated monocytes. The vaccine was also without a stimulatory or modulatory effect upon TNF-α or IL-1β secretion by monocytes from a patient with the AIDS-related complex (ARC) and from the monocytic THP-1 cell line. The lack of effect of gp 160 on monokine production has important implications for its efficacy as a vaccine for AIDS.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Feb 01, 1991
- Source ID
- 10.1111/j.1365-2249.1991.tb05613.x
Entities
People
- D P Dooley
- D. J. Looney
- R. A. Cox
Organizations
- San Antonio Military Medical Center
- Walter Reed Army Institute of Research