Acute motor deficit and subsequent remyelination‐associated recovery following internal capsule demyelination in mice
Abstract
Multiple sclerosis is a chronic inflammatory demyelinating disease of the central nervous system (CNS), characterized by accumulated motor disability. However, whether remyelination promotes motor recovery following demyelinating injury remains unclear. Damage to the internal capsule (IC) is known to result in motor impairment in multiple sclerosis and stroke. Here, we induced focal IC demyelination in mice by lysophosphatidylcholine (LPC) injection, and examined its effect on motor behavior. We also compared the effect of LPC‐induced IC damage to that produced by endothelin‐1 (ET1), a potent vasoconstrictor used in experimental stroke lesions. We found that LPC or ET1 injections induced asymmetric motor deficit at 7 days post‐lesion (dpl), and that both lesion types displayed increased microglia/macrophage density, myelin loss, and axonal dystrophy. The motor deficit and lesion pathology remained in ET1‐injected mice at 28 dpl. In contrast, LPC‐injected mice regained motor function by 28 dpl, with corresponding reduction in activated microglia/macrophage density, and recovery of myelin staining and axonal integrity in lesions. These results suggest that LPC‐induced IC demyelination results in acute motor deficit and subsequent recovery through remyelination, and may be used to complement future drug screens to identify drugs for promoting remyelination.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Aug 14, 2020
- Source ID
- 10.1111/jnc.15142
Entities
People
- Jeffrey K Huang
- Nobuhiko Ohno
- Reiji Yamazaki
Organizations
- Congressionally Directed Medical Research Programs
- Georgetown University
- Jichi Medical University
- National Center of Neurology and Psychiatry
- National Institutes of Health
- National Multiple Sclerosis Society
- SENSHIN Medical Research Foundation