Spectral Properties of Fluorogenic Thiophene‐derived Triarylmethane Dyes
Abstract
Spectral properties and fluorogenic behaviors of five novel thiophene variants of malachite green (MG), termed MGTs, were determined. Appreciable changes as a function of homologation and substitution pattern, including absorption band positions and intensities and fluorescence quantum yields were observed. In particular, the shorter wavelength y‐band absorption was found to shift over a nearly 200 nm range based on aryl group variation, allowing fine‐tuning of the excitation wavelength for these dyes. In addition, the fluorescence intensity of some MGTs increased significantly (up to 4600‐fold) when the dye was bound to a cognate protein partner, which is potentially useful for cell imaging studies.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Nov 23, 2018
- Source ID
- 10.1111/php.13037
Entities
People
- Alan S. Waggoner
- Bruce A. Armitage
- Kenneth F. Stensrud
- Kimberly J. Zanotti
Organizations
- Air Force Office of Scientific Research
- Carnegie Mellon University
- National Institutes of Health
- National Science Foundation
- United States Department of Defense