Combining one‐step Sanger sequencing with phasing probe hybridization for HLA class I typing yields rapid, G‐group resolution predicting 99% of unique full length protein sequences
Abstract
Sanger‐based DNA sequencing of exons 2+3 of HLA class I alleles from a heterozygote frequently results in two or more alternative genotypes. This study was undertaken to reduce the time and effort required to produce a single high resolution HLA genotype.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jan 18, 2017
- Source ID
- 10.1111/tan.12951
Entities
People
- Bin Tu
- Carly Masaberg
- Carolyn Katovich Hurley
- Daniel Behm
- Jar How Lee
- Jennifer Ng
- John Sells
- Kanthi Kariyawasam
- Lihua Hou
- Nuri Cha
- Paul Tausch
- Peter Brescia
- Ruyan Yang
- Thoa Nong
Organizations
- Georgetown University
- Office of Naval Research
- Thermo Fisher Scientific Inc.